Abstract In pot experiments the effect of a temporary infestation of maize by Chenopodium album L. on water and nitrogen leaching after a simulated strong rainfall was tested for a silty loam and a loamy sand. The results show that the leaching of 15N under the initial stage of maize can be reduced considerably by a temporarily weed infestation. Neither yield of maize nor uptake of nitrogen until corn maturity were reduced on both a silty loam and a loamy sand. 相似文献
Atrazine is one of the most frequently detected pesticides and poses a great risk to humans and the environment. In this study, the effects of two submerged macrophytes, Potamogeton crispus and Myriophyllum spicatum, on phytoremediation of atrazine were evaluated. The results showed that atrazine decayed faster in the pots where these two plants were cultivated than that of the control without the plants during the 60-day course of the experiment. Metabolite analyses of atrazine indicated that atrazine was mainly converted to diaminochlorotriazine and hydroxyatrazine. Dissipation kinetics and risk assessment of atrazine showed that atrazine was degraded rather fast in maize and generally safe for human consumption at the recommended high dosage of atrazine. The maximum permissible intake (MPI) for atrazine was calculated to be 0.23 μg/person/day for a child and 0.72 μg/person/day for an adult. However, due to the large-scale application of atrazine over the years, the atrazine residue still poses a great threat to the environment. By using submerged macrophytes M. spicatum and P. crispus, atrazine could be absorbed from the sediment, hence remediating contaminated sediment and water. This study established a protocol for evaluating submerged plants in absorption or phytoremediation of pesticides. 相似文献
The synthesized full-length cDNA to CMV satellite RNA-1 was integrated into plant expression vector RoKII with a CaMV 35S promoter. Infected by Agrobacterium tumefaciens harbouring the recombinant plasmid. The tobacco leaf discs (the G-140 variety which is widely cultivated in China) were regenerated into plants. After being inoculated with virulent strain CMV, most of the transgenic plants expressed satellite RNA at high levels and developed a much milder symptom than the untransformed ones.Basically in accordance with Mendel's law of segregation, the novel tobacco pure line engineered with viral resistance was screened out. The secondary generation transgenic plants still maintained high level satellite RNA expression and the resistance to CMV. 相似文献
In this study, ten Fusarium toxins were analysed in wheat and maize commodities from Albania. In total, 71 samples of wheat and 45 samples of maize were collected from different producing regions. The analytical procedure consisted of a simple one-step sample extraction followed by the determination of toxins using liquid chromatography coupled with tandem mass spectrometry. Fusarium toxins were found in 23% of the analysed wheat samples and in 78% of maize samples. In maize samples, most often fumonisins B1 (FB1) and B2 (FB2) were found. They were present in 76% of samples. They were detected in all positive samples except in one with concentrations ranging from 59.9 to 16,970 μg/kg. The sum of FB1 and FB2 exceeded the EU maximum permitted level (4000 μg/kg) in 31% of maize samples. In wheat samples, the only detected Fusarium mycotoxin was deoxynivalenol (DON), present in 23% of samples. In one sample with the concentration of 1916 μg/kg, the EU maximum permitted level (1250 μg/kg) was exceeded. This is the first report on the presence of Fusarium toxins in wheat and maize grains cultivated in Albania. 相似文献
The concentrations of 19 elements in wild and genetically modified Nicotiana langsdorffii (N. langsdorffii) exposed to Chromium (VI) and to water deficit were determined and compared to provide new information about their response to abiotic stress.
Genetic modifications by GR and RolC genes (encoding for the rat glucocorticoid receptor and for Agrobacterium rhizogenes RolC, respectively) were investigated because they induce significant, but only partially known changes in the plant response to stress.
Simultaneous determination of Al, As, Ba, Ca, Cd, Co, Cr, Cs, Cu, Fe, K, Mg, Mn, Na, Pb, Rb, Sr, V and Zn was carried out by ICP-MS equipped with a collision/reaction cell (ICP-ORS-MS). The methodology was optimised by testing the grinding, homogenisation, digestion and analysis procedures, to reduce the uncertainty of the experimental results and to identify statistically significant differences between nine sample pools, for a total of 75 samples. The quality control procedure was carried out by blank control and by evaluating the detection limits and repeatability. Trueness was assessed by analysing certified reference material, NIST 1573a.
Significant differences were observed in the uptake and accumulation of several elements in the wild-type N. langsdorffii samples, either with respect to the plants submitted to water deficit and exposure to Cr(VI) or with respect to the genetically modified plants. The differences were highlighted by principal component analysis (PCA). The analysis of the element content of the whole plant, combined with the data found in the literature, allows us to hypothesise effects on the metabolic mechanism controlling the uptake and translocation of elements inside the vegetal organism. Because genetic and chemical stress decreases the nutrient concentration in the whole plant, we can say that the uptake at root level is affected. The increase in concentration of elements such as As, Sr and Al indicates a decreased selectivity in the uptake of potentially toxic elements and, consequently, highlights the effects on the plant’s metabolic processes. 相似文献
Transgenic Catharanthus roseus plants (transgenic Dhawal [DT] and transgenic Nirmal [NT]) obtained from the Agrobacterium tumefaciens and Agrobacteriumrhizognenes-mediated transformations, respectively, have been maintained in vitro for 5 years. Plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. DT plant gradually lost the GUS gene expression and it was not detected in the fifth year while NT plant demonstrated the presence of genes rolA, rolB and rolC even in the fifth year, indicating the more stable nature of Ri transgene. Vindoline content in the DT was two times more than in non-transformed control plants. Alkaloid and tryptophan profiles were almost constant during the 5 years. The cluster analysis revealed that the DT plant is more close to the control Nirmal plant followed by NT plant. 相似文献